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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Protein Expr Purif. 2008 Aug 15;62(2):171–178. doi: 10.1016/j.pep.2008.08.002

Fig. 3.

Fig. 3

A, Coomassie-stained denaturing gel electrophoresis showing near complete incorporation of hSCD1 (black star) into synthetic liposomes from wheat germ cell-free translation. Proteoliposomes were loaded underneath a discontinuous Accudenz gradient and floated by ultracentrifugation. Bound protein, including hsp70 (white star) and hSCD1 floated (lanes 2 and 3), while unbound protein remained at the bottom (lanes 8–10). B, a comparison of proteoliposomes from floated fraction 2 containing hSCD1 (lane 1), cytb5 (lane 2), and a co-translation of hSCD1 and cytb5 (lane 3, 1 eq of hSCD1 mRNA with 2 eq of cytb5 mRNA; lane 4, 1 eq of hSCD1 mRNA with 4 eq of cytb5 mRNA).