Table 1. Analysis of p53 and p19ARF sequence in tumor samples.
Exons 5–10 of p53 genomic DNA and exons 2–11 of p53 cDNA were PCR amplified and sequenced. The coding sequence of p19ARF was amplified and sequenced (exons 1–3) from cDNA samples. All sequencing was performed multiple times and PCR products were sequenced in both directions.
p53 exon amplification from genomic DNA | ||||||||
---|---|---|---|---|---|---|---|---|
ID | genotype | tumor type | Ex. 5 | Ex. 6 | Ex.7 | Ex. 8 | Ex. 9 | Ex. 10 |
22790 | M-C2 | basal carcinoma | WT | WT | WT | WT | WT | WT |
23186 | N-C2 | adeno/squamous carcinoma | WT | WT | WT | WT | WT | WT |
23182 | M-C2 | osteosarcoma | WT | WT | WT | WT | WT | WT |
23828 | N-C2 | granulosa cell tumor | WT | WT | WT | WT | WT | WT |
21872 | N-C2 | histiocytic sarcoma | WT | WT | WT | WT | WT | WT |
23646 | N-C2 | lymphoma | WT | WT | WT | WT | WT | WT |
23649 | N-C2 | mammary carcinoma | WT | WT | WT | WT | WT | ND |
24098 | N-C2 | basal carcinoma | WT | WT | WT | WT | WT | WT |
p53 and p19ARF amplification from cDNA | ||||
---|---|---|---|---|
ID | tumor type |
p53 Cdna exons 2–11 |
p19ARF cDNA exons 1–3 |
|
23902 | M-C2 | basal carcinoma | WT | NA |
24071 | N-C2 | lymphoma | WT | NA |
23828 | N-C2 | granulosa cell tumor | WT | WT |
23129 | M-C2 | histiocytic sarcoma | WT | WT |
23186 | N-C2 | adeno/squamous carcinoma | WT | WT |
25094 | N-C2 | uterine leiomyosarcoma | WT | WT |
23646 | N-C2 | lymphoma | WT | WT |
(M-C2=Mre11ATLD1/ATLD1 Chk2−/−, N-C2=Nbs1ΔB/ΔB Chk2−/−, WT=wild type, ND=not determined, NA=not amplifiable)