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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: Gen Comp Endocrinol. 2007 Jan 4;151(1):90–97. doi: 10.1016/j.ygcen.2006.12.023

Fig. 1.

Fig. 1

Primer validation. (a) Primers for zfMSTN-1, -2 and EF1-α were used to amplify adult zebrafish skeletal muscle cDNA using the annealing temperatures indicated. (b) Assessment of potential primer cross-reactivity. Plasmids containing cDNA for each myostatin were amplified with specific or non-specific primers (1/2 = zfMSTN-1 plasmid + zfMSTN-2 primers, etc.).