Qualitative assessement of zfMSTN-1, -2 and EF1-α expression throughout embryogenesis. Levels of each transcript were qualitatively assessed by RT-PCR using embryos collected at the indicated times (hpf, hours post-fertilization). Adult RNA was extracted from skeletal muscle. (NTC, no template control; 11.3 hpf, 4 somite; 13, 8 somite; 15.5, 13 somite; 17.5, 17 somite; 19, 20 somite; 21.5, 25 somite).