Table 11.
Repetitive dilution verification comparison between wrist shaker and sonication for Aristolochia spp. root diluted with S. tetrandra root
| Aristolochic acid concentration, μg/g
|
|||||
|---|---|---|---|---|---|
| Extraction interval | Extraction time, min | Replicate 1 | Replicate 2 | Replicate 3 | Mean |
| Wrist shaker
| |||||
| Initial | 30 | 610 | 626 | 635 | 624 |
| After 1st dilution | 30 | 619 | 617 | 642 | 626 |
| After 2nd dilution | 30 | 611 | 664 | 679 | 651 |
| Overall mean | 634 | ||||
| Overall SDa | 15.0 | ||||
| Overall RSDrb | 2.37 | ||||
| Sonication
| |||||
| Initial | 30 | 599 | 599 | 610 | 603 |
| After 1st dilution | 30 | 583 | 610 | 589 | 594 |
| After 2nd dilution | 30 | 586 | 641 | 649 | 625 |
| Overall mean | 607 | ||||
| Overall SD | 15.9 | ||||
| Overall RSDr | 2.62 | ||||
a
SD = Standard deviation.
b
RSDr = % Relative standard deviation, intralaboratory precision.