Table 15.
Test material precision
| Aristolochic acid concn, μg/g
|
Days 1–3 combined
|
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|---|---|---|---|---|---|---|---|---|---|
| Replicate | Day 1 | Day 2 | Day 3 | Mean, μg/g | SDa | RSDrb | PRSDRc | HorRatd | Target RSDre |
|
Aristolochia spp. root
| |||||||||
| 1 | 1410 | 1410 | 1410 | 1450 | 47.3 | 3.26 | 5.33 | 0.6 | 2.67–3.56 |
| 2 | 1490 | 1490 | 1420 | ||||||
| 3 | 1490 | 1520 | 1400 | ||||||
| Mean | 1460 | 1470 | 1410 | ||||||
| SD | 46.2 | 56.9 | 10.0 | ||||||
| RSDr | 3.16 | 3.87 | 0.709 | ||||||
|
| |||||||||
|
Aritolochia manschuriensis stem
| |||||||||
| 1 | 2890 | 2790 | 2860 | 2830 | 85.3 | 3.01 | 4.82 | 0.6 | 2.41–3.21 |
| 2 | 2900 | 2940 | 2680 | ||||||
| 3 | 2820 | 2850 | 2720 | ||||||
| Mean | 2870 | 2860 | 2750 | ||||||
| SD | 43.6 | 75.5 | 94.5 | ||||||
| RSDr | 1.52 | 2.64 | 3.44 | ||||||
|
| |||||||||
|
Akebia trifoliate stem
| |||||||||
| 1 | <2.00 | <2.00 | <2.00 | <2.00 | NAf | NA | NA | NA | NA |
| 2 | <2.00 | <2.00 | <2.00 | ||||||
| 3 | <2.00 | <2.00 | <2.00 | ||||||
| Mean | <2.00 | <2.00 | <2.00 | ||||||
| SD | NA | NA | NA | ||||||
| RSDr | NA | NA | NA | ||||||
|
| |||||||||
|
Clematis armandii stem, 20 μg/g fortification with, Aristolochia spp.
| |||||||||
| 1 | 23.4 | 22.4 | 22.6 | 22.5 | 0.550 | 2.44 | 9.96 | 0.2 | 4.98–6.64 |
| 2 | 23.2 | 22.0 | 22.1 | ||||||
| 3 | 22.4 | 21.7 | 22.7 | ||||||
| Mean | 23.0 | 22.0 | 22.5 | ||||||
| SD | 0.529 | 0.351 | 0.321 | ||||||
| RSDr | 2.30 | 1.60 | 1.43 | ||||||
|
| |||||||||
|
S. tetrandra root:, 10 μg/g fortification with Aristolochia spp.
| |||||||||
| 1 | 10.0 | 11.6 | 10.1 | 10.5 | 0.776 | 7.39 | 11.2 | 0.7 | 5.60–7.47 |
| 1 | 10.5 | 10.0 | 11.0 | ||||||
| 3 | 11.8 | 9.82 | 9.76 | ||||||
| Mean | 10.8 | 10.5 | 10.3 | ||||||
| SD | 0.929 | 0.980 | 0.641 | ||||||
| RSDr | 8.60 | 9.33 | 6.22 | ||||||
|
| |||||||||
| Tablets, 10 μg/g fortification with Aristolochia spp.
| |||||||||
| 1 | 6.55 | 6.83 | 6.09 | 6.50 | 0.450 | 6.92 | 12.0 | 0.6 | 6.00–8.00 |
| 2 | 6.93 | 5.87 | 5.93 | ||||||
| 3 | 6.43 | 6.76 | 7.10 | ||||||
| Mean | 6.64 | 6.49 | 6.37 | ||||||
| SD | 0.261 | 0.535 | 0.634 | ||||||
| RSDr | 3.93 | 8.24 | 9.95 | ||||||
|
| |||||||||
| Tablets, 20 μg/g fortification with Aristolochia spp.
| |||||||||
| 1 | 16.7 | 18.8 | 16.6 | 17.2 | 1.42 | 8.26 | 10.4 | 0.8 | 5.20–6.94 |
| 2 | 15.0 | 15.7 | 19.1 | ||||||
| 3 | 17.0 | 17.7 | 18.6 | ||||||
| Mean | 16.2 | 17.4 | 18.1 | ||||||
| SD | 1.08 | 1.57 | 1.32 | ||||||
| RSDr | 6.67 | 9.02 | 7.29 | ||||||
a
SD = Standard deviation.
b
RSDr = % Relative standard deviation, intralaboratory precision.
c
PRSDR = 2 C−0.05, % relative standard deviation, predicted interlaboratory precision, C = mean concentration expressed as a mass fraction.
d
HorRat = RSDr/PRSDR.
e
Target RSDr = % Acceptable intralaboratory precision (0.500 to 0.667) PRSDR.
f
NA = Not applicable.