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Representative MALDI-TOF MS spectrum of a selected lysine peptide library bead. The binary tag system allowed unambiguous identification of the Gln, Val, and Leu residues and the full-length peptide Gln-Tyr-Lys-Val-Leu. The peak at m/z 1818.91 was assigned to Gln, which is known to undergo cyclization during MALDI when present at the N-terminal position, resulting in a mass loss of m/z 17.
