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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: J Immunol. 2008 Jul 15;181(2):1357–1364. doi: 10.4049/jimmunol.181.2.1357

Figure 3. Effects of IL-17R deficiency on the number of IL-17A-producing Tn cells and IL-17A secretion.

Figure 3

Splenocytes from Il17ra−/− and WT mice were stimulated for 3 days on plate-adsorbed anti-CD3ε and soluble anti-CD28 antibodies in the presence or absence of IL-23 (20 ng/ml), followed by a 3 day rest. Cells were reactivated by PMA/ionomycin in the presence of GolgiStop for 5 h before intracellular staining for IL-17A. (A) Representative plots from WT and Il17ra−/− mice. (B) IL-17A-producing Tn cells (n=3–4) as a proportion (%) of the gated lymphocyte population in Il17ra−/− splenocytes (open bars) compared to WT splenocytes (filled bars). (C) IL-17A protein in the supernatants (n=3–5). *p<0.05 by unpaired t test.

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