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Induction of apoptosis of Jurkat cell by Amph-2. The cells were untreated (Ctrl) or treated with 0.5 or 1.0 µg/ml of Amph-2, or with 1.0 µg/ml of the alkylated form of Amph-2 (Amph alk) for 72 h, then fixed and the presence of DNA strand breaks was detected by the TUNEL assay,26,32 DNA was counterstained with DAPI. The cells with DNA strand breaks above the threshold marked by the dashed line were classified as apoptotic and their percent is shown (Ap).
