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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: Cell Cycle. 2007 Sep 12;6(24):3097–3102. doi: 10.4161/cc.6.24.5045

Figure 5.

Figure 5

Induction of apoptosis (activation of serine proteases) of HL-60 cells treated with Amph 1-4 and by Onc. The cells were untreated (Ctrl) or treated with 1 µg/ml of Amph 1-4 or 5 µg/ml of Onc for 72 h, then incubated with the fluorochrome—(F; carboxyfluorescein)—tagged inhibitor and active center ligand of chymotrypsin-like proteases (FFCK) and with PI, and their green and red fluorescence was measured by flow cytometry as described in reference 28. Activation of serine proteases is detected by binding of FFCK above the background level marked by horizontal dashed line.28 As in Figure 4, four cell subpopulations, representing live cells (A) and cells at various stages of apoptosis (B-D), can be discriminated based on binding FFCK and exclusion of PI. The events representing cell debris (Fig. 4,”E”) were gated out.