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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: Dev Dyn. 2008 May;237(5):1359–1372. doi: 10.1002/dvdy.21536

Figure 7. Disruption of the roof plate and dorsal midbrain gene expression patterns in the absence of SHH.

Figure 7

A, B, E, F. Free floating embryos shown in dorsal view with rostral to the top. C, D. Midbrain cross-sections with dorsal to the top. G-J: wholemounts in sagittal view, rostral to the right. A-F. Broadening and disruption of the RP markers, Gdf7 (A-D) and Wnt1 (E, F) in the Shh-/- mouse (B, D, F) compared to wild-type littermates (A, C, E). Dashed lines in C and D indicate the ventral outline of the midbrain. Arrows in F point to ectopically scattered Wnt1+ cells. G, H. Expression of Lfng at E9.5 in the Shh-/- midbrain (H) is reduced compared to control littermate (G). I, J. Increased spread and reduced intensity of the dorsal marker Zic1 in the E10.5 Shh-/- mouse (J), compared to controls (I). Arrowheads in H and J delineate the midbrain from the hindbrain in the Shh-/- mice. Abbreviations: DMB: diencephalic-midbrain boundary; HB: hindbrain; MB: midbrain; MHB: midbrain-hindbrain boundary; RP: roof plate.