Table 1. Total numbers of TUNEL+ and Evx1+ neurons in wild-type and Shh-/- midbrains.
| Embryonic Age | Mean Cell Count/brain (WT) | Mean Cell Count/brain (Shh-/-) | % Difference* | p Value** | |
|---|---|---|---|---|---|
| TUNEL+ cells | E8.5 | 86.7 ± 5.5 | 196.7 ± 16.0 | 126.9% | <0.01 7 |
| E9 | 113.3 ± 11.6 | 218.0 | 92.4% | <0.023 | |
| Evx1+ cells | E12.5 | 11,631.4 ± 1,587.2 | 3055.9 ± 283.7 | -73.7% | <0.014 |
*
Percent Difference was calculated as follows: (wild-type total cell number - Shh-/- total cell number)/wild-type midbrain x 100. (For details, see Materials and Methods)
**
determined by Student’s t test.