Table 1.
MEFs | IC50 (nM) | ||
---|---|---|---|
vinorelbine | cisplatin | doxorubicin | |
RLIP76+/+ | 2.2 ± 0.31 | 1520 ± 180 | 110 ± 20 |
RLIP76+/− | 1.3 ± 0.15 | 830 ± 110 | 42 ± 8 |
RLIP76−/− | 0.4 ± 0.03 | 360 ± 50 | 23 ± 3 |
RLIP76-liposomes treated RLIP76−/− | 1.8 ± 0.22 | 1270 ± 185 | 86 ± 11 |
Drug sensitivity assays were performed using MTT (at 96 h) to determine IC50 values [23]. The values are presented as mean ± SD from three separate determinations with eight replicates each (n =24). Statistical analyses by ANOVA were significant at p< 0.01 for RLIP76+/+ vs. RLIP76+/−, RLIP76+/+ vs. RLIP76−/−, RLIP76+/− vs. RLIP76−/− and RLIP76−/− vs. RLIP76-proteoliposomes treated RLIP76−/−,