Skip to main content
. Author manuscript; available in PMC: 2009 Jun 11.
Published in final edited form as: J Am Chem Soc. 2008 May 14;130(23):7357–7363. doi: 10.1021/ja077863d

Figure 2.

Figure 2

(a) SDS-gel electrophoresis of OmpX samples at pH 8.5. Lane 1: unfolded OmpX in 6 M urea; Lane 2: OmpX refolded in DDM; Lane 3: OmpX refolded in TPC. (b) pH effect on OmpX refolding. F is the fraction of the OmpX that was refolded. The protein (0.4 mg/mL), urea (0.2 M), and detergent (1% wt/vol) were incubated at room temperature for 3 hours, followed by quick analysis by SDS-PAGE. The fraction of folded OmpX was determined by densitometric analysis of the Coomassie stained gels (± 5% error). The relatively high concentration ensured that each detergent was present above CMC; the long incubation time ensured completion of the refolding process before the start of each measurement.44