. Author manuscript; available in PMC: 2009 Jun 11.

Published in final edited form as: J Am Chem Soc. 2008 May 14;130(23):7357–7363. doi: 10.1021/ja077863d

Table 3.

Micro-scale OmpX reconstitution with different detergents.

Detergent	OmpX precipitation in refolding buffer^a	OmpX precipitation in NMR buffer^b	NMR Quality^c
DHPC	∼30%	∼40%	++
138-Fos	0%	0%	++
179-Fos	0%	0%	+
115-Fos	0%	0%	−
TPC	0%	0%	−
34-Fos	0%	0%	−
185-Fos	0%	0%	−

Recoverable OmpX loss due to precipitation during refolding.

Recoverable OmpX loss due to buffer exchange (Figure 1).

2D [¹⁵N,¹H]-TROSY spectra (Figure 4) rated “++” for spectra suitable for a structure determination, “+” for spectra with detergent artifacts, and “-” for spectra showing that OmpX was solubilized, but not reconstituted.