Figure 2. Binding of recombinant B. burgdorferi proteins to selected integrins.

Integrins at 3 μg/ml were immobilized in plastic 96 well plates. The buffer alone was also plated as a control. After blocking non-specific protein binding sites, the recombinant proteins at 10 μg/ml were added to the wells and incubated for 1.5 hours at ambient temperature. Unbound proteins were removed by washing and the plates were fixed, blocked, and protein binding was detected by ELISA using an anti-MBP antiserum. Shown are the means plus standard deviations of 4 replicates, after subtraction of binding to control wells with no integrin, from one representative of at least 3 independent experiments for each protein.