Figure 3. RGD peptide Inhibition of MBP-BBB07 binding to α₃β₁.

Wells coated with the integrin at 3 μg/ml were incubated with varying concentrations of the peptides for 30 min. at ambient temperature, then MBP-BBB07 was added to 10 μg/ml. Binding was quantified by ELISA as described for Figure 2, then normalized to the level of binding in the absence of peptide. Shown are the means and standard deviations of 4 replicates from one representative experiment. In all experiments, binding of MBP was negligible and not affected by the peptides (not shown). Statistically significant (by the two tailed t test, p<0.05) inhibition of BBB07 binding in comparison to either the absence of peptide or GRGESPK was seen only with 2 mg/ml GRGDSPK.