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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: Cell Microbiol. 2007 Sep 6;10(2):320–331. doi: 10.1111/j.1462-5822.2007.01043.x

Figure 4. Inhibition of B. burgdorferi attachment to integrin α3β1 by recombinant MBP-BBB07 and MBP-P66M.

Figure 4

Integrin α3β1 plated at 3 μg/ml was incubated for 30 min. with the recombinant proteins at the concentrations indicated prior to the addition of B. burgdorferi strain N40. Bacterial attachment was quantified by ELISA using an anti-Lyme spirochete rabbit antiserum followed by alkaline phosphatase-conjugated anti-rabbit IgG. Shown are the means and standard deviations of 4 replicates, representing two independent experiments for MBP and P66M, which previously showed similar curves with both β3 chain integrins (Coburn et al., 1999), and four independent curves for BBB07.