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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: Cell Microbiol. 2007 Sep 6;10(2):320–331. doi: 10.1111/j.1462-5822.2007.01043.x

Figure 6. B. burgdorferi protein BBB07 induced expression of proinflammatory cytokines, chemokines and matrix metalloproteinases (MMPs) is mediated by integrin α3 in human chondrocyte cells.

Figure 6

Primary HC cells were transfected with either human integrin α3 siRNA or control siRNA and at 24 h posttransfection treated with either MBP-BBB07 or MBP-P66 for 8h. All incubations included 50 μg/ml polymyxin B. Total cellular RNA was isolated, and q-RT-PCR was done. The relative expression of each treatment group is compared with siControl+BBB07 group, which is normalized to one. The experiment was repeated four times, and average of the all experiments is shown. Error bars represent standard deviations. The siControl had no effect on gene expression in response to either recombinant protein in comparison to either treatment with lipofectamine alone or no treatment with transfection reagents (data not shown).