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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: Anticancer Res. 2008;28(3A):1441–1447.

Figure 3.

Figure 3

HIV-1 Tat stimulates Rad51 promoter activity and homologous recombination. A. MEFs were transfected with pGL3Rad51-luciferase plasmid along with pcDNA3-vector, pcDNA3-Purα or pcDNA-Tat expression plasmid using Lipofectamine (Invitrogen). Luciferase activity was determined after 36 h. The data represent results from three independent experiments. B. The effects of HIV-1 Tat and Pura on homologous recombination-directed DNA repair were examined. PURA-/- and PURA-/- (+Purα) MEFs were transfected with pcDNA3 or pcDNA3-TAT along with pCMV-3nls-I-SceI for 48 hours. The effect of HIV-1 TAT in PURA-/- (+Purα) cells was significantly different from control, *p<0.01, but that in PURA-/- cells was not, **p>0.05.