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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: Eur J Immunol. 2008 Jul;38(7):1988–1998. doi: 10.1002/eji.200737738

Figure 5.

Figure 5

Enhanced trafficking of Gαi3-deficient T effectors to inflammatory sites in early GVHD hosts. Gαi2- or Gαi3-KO T cells and WT T cells activated by MHC-mismatched hosts were isolated, stained with a fluorescent dye TRITC or CFSE, respectively, mixed at a 1:1 ratio, and injected into SCID mice that had been primed by WT T cells for three weeks as detailed in the Materials and methods. Distribution of Gαi2- or Gαi3-KO T cells relative to WT T cells in different tissues was analyzed at 1 or 18 h after T cell transfer. Homing efficiency in the presence or absence of a specific Gαi protein is expressed as homing indices where homing index for WT T cells is normalized to 1 (dash line). Data shown are the means ± SD for seven mice at each time point in two separate experiments. BM, bone marrow; PLN, peripheral lymph nodes; MLN, mesenteric lymph nodes; *p<0.05, **p<0.01 compared to WT control T cells.