Fig. 5. Polytene chromosome labeling by three different H3S10ph antibodies in response to heat shock treatment in JIL-1 null mutants.

Acid-free polytene chromosome squash preparations from female JIL-1^z2/JIL-1^z2 third instar larvae triple labeled with antibodies to Pol IIo^ser2 (in green), H3S10ph (in red), and with Hoechst (DNA, blue/grey) after heat shock treatment. H3S10ph labeling by antibodies from Cell Signaling, Epitomics, and Upstate are shown. Arrows indicate the likely position of the 87A/C heat shock puff regions.