Fig. 6. Immunoblot analysis of histone H3S10 phosphorylation in mutants of the 55 kd regulatory subunit of protein phosphatase 2A.

(A) H3S10ph antibody labeling of protein extracts from wild type (wt) and tws⁰²⁴¹⁴/tws⁰²⁴¹⁴ (tws⁰²⁴¹⁴) whole third instar larvae. (B) H3S10ph antibody labeling of protein extracts from wild type (wt) and tws⁰²⁴¹⁴/tws⁰²⁴¹⁴ (tws⁰²⁴¹⁴) salivary glands. (C) H3S10ph antibody labeling of protein extracts from tws^P/tws^P (tws^P) and JIL-1^z2/JIL-1^z2 (z2) salivary glands with (HS) and without heat shock treatment. The H3S10ph antibody was from Epitomics (epi) and labeling with histone H3 (H3) antibody was used as a loading control.