Figure 4. Effect of PDGF-BB concentration and growth factor to heparin ratio on the rate of fibrin matrix degradation by tendon fibroblasts.
Fibrin matrices containing HBDS and PDGF-BB were washed with 1 mL of TBS five times for 24 h and then seeded with 50,000 tendon fibroblasts per matrix. The media was collected every 24 h for the following 9 days and the fibrinogen in the media or TBS was quantified by measuring the fluorescently labeled fibrinogen in solution. ‘*’ denotes p<0.001 versus 0.125 μg/mL of PDGF-BB with or without delivery system over time.