Figure 5.

DNA extracted from the cerebella of Tg(PG14) mice displays a 200-bp ladder. Detergent extracts of cerebella were centrifuged at 16,000 × g, and DNA extracted from pellet fraction (lanes 1, 3, 5, and 7) and supernatant fraction (lanes 2, 4, 6, and 8) was subjected to Southern blotting using restriction-digested mouse genomic DNA as a probe. Samples were from mice of the following ages: 182 days (lanes 1 and 2), 187 days (lanes 3 and 4), 178 days (lanes 5 and 6), and 176 days (lanes 7 and 8). One-thirtieth of the DNA extracted from the pellet fractions, and the whole amount of DNA extracted from the supernatant fractions were analyzed. Size markers are given in base-pairs.