FIGURE 4. Homing and functional properties of SV40_IV-TCR modified T cells.

10-week old TRAMP mice and control non-transgenic littermates received an adoptive transfer of 5×10⁵ SV40_IV-TCR transduced T cells, followed by vaccination by i.p. infection with 1×10⁵ p.f.u. of rVV-T. Control mice were solely vaccinated with rVV-T. 11 days post vaccination, the frequency of TCR transduced cells in spleen and prostate was assessed by analysing the percentage of Vβ9⁺Vβ-pool⁺ CD8⁺ cells of total Vβ-pool⁺ CD8⁺ cells. Functionality of SV40_IV-specific T cells was measured by intracellular IFN-γ staining after incubation for 4 hours with 100 ng/ml of the relevant peptide (SV40_404-411) or control peptide (OVA_257-264). Shown are dot-plots from a mouse in each of the treatment groups (A) and cumulative data from all mice (B). Numbers in upper right corner of dot-plots refer to percentage of Vβ9⁺Vβpool⁺ cells of total CD8⁺ cells or IFNγ⁺CD8⁺ cells of total CD8⁺ cells. Circles in graphs represent individual mice, bars indicate averages.