Table 1.
Purification of rhTNSALP and acidic oligopeptide-tagged TNSALP from condition medium
| Protein conc. (mg/l) | Total protein (mg) | Total enzyme activity (U) | Specific activity (U/mg) | Purification | Yield (%) | |
|---|---|---|---|---|---|---|
| rhTNSALP | ||||||
| Crude media | 5.26 | 115 | 3003 | 26.1 | 1 | 100 |
| DEAE column | 18.3 | 0.66 | 1555 | 2354 | 90 | 52 |
| Sephacryl S-400-HR column | 15.4 | 0.35 | 973 | 2744 | 105 | 32 |
| CD6-TNSALP | ||||||
| Crude media | 6.27 | 127 | 3022 | 23.9 | 1 | 100 |
| DEAE column | 32.3 | 1.01 | 2073 | 2043 | 86 | 69 |
| Sephacryl S-400-HR column | 22.1 | 0.77 | 1862 | 2411 | 101 | 62 |
| CD8- TNSALP | ||||||
| Crude media | 3.85 | 184 | 3065 | 16.6 | 1 | 100 |
| DEAE column | 29.1 | 1.00 | 2028 | 2035 | 123 | 66 |
| Sephacryl S-400-HR column | 22.4 | 0.72 | 1702 | 2374 | 143 | 56 |