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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: Mol Genet Metab. 2006 Apr 17;88(3):244–255. doi: 10.1016/j.ymgme.2006.02.012

Table 3.

Binding parameters of three enzymes to hydroxyapatite

Kd (U/ml) Bmax (U/100 μg hydroxyapatite)
rhTNSALP 1.66 ± 0.86 1.0 ± 0.3
CD6-TNSALP 0.059 ± 0.017 3.3 ± 0.8
CD8-TNSALP 0.044 ± 0.005 3.4 ± 0.9

Each value represents means ± SD of three experiments. Kd, dissociation constant, and Bmax, maximal binding rate, were determined from double-reciprocal plots.