TABLE 3.
Distribution of Mutated K-ras DNA in Fractionated Urine DNA in Two Patients
| Patient A
|
Patient B
|
|||
|---|---|---|---|---|
| Fractions | DNA concentration
(ng/μL) |
Mutated K-ras DNA | DNA concentration
(ng/μL) |
Mutated K-ras DNA |
| <150 bp | ND | + | ND | + |
| 150–300 bp | 0.010 | + | 0.016 | + |
| 300–500 bp | 0.013 | + | 0.043 | + |
| 500–700 bp | 0.024 | + | 0.043 | + |
| 700 bp–1 kb | ND | + | 0.013 | − |
| 1–1.5 kb | ND | − | 0.010 | − |
| 1.5–2 kb | 0.016 | − | ND | − |
| 2–3 kb | ND | − | ND | − |
| 3–4 kb | ND | − | ND | − |
| 4–10 kb | ND | − | ND | − |
| 10 kb–well | 0.03 | + | ND | + |
DNA concentration was determined by the real-time PCR assay using K-ras Q-primers as described in Materials and Methods. The limit of detection of the assay is 0.010 ng/μL/rxn; ND: below the limit of detection.
Mutated K-ras DNA was detected by RE-PCR (≥2copies per reaction). The positive sign (+) shows that the mutated DNA was detected and the negative (−) indicates that the mutated K-ras DNA was not detected.