Table 1. Mutations that could disrupt the α-helical coiled-coil structure of the rod.
Mutations were analyzed using both Paircoil and Coils algorithms. Paircoil predictions were generated with the Probability Cutoff equal to 0.5. Coils predictions were generated with two different coiled-coil database derived scoring matrixes (MTK, MTDK) and three scanning amino acid windows (14, 21, 28 amino acid). +/− corresponds to mutations having a mild disruptive effect on the predicted coiled-coil structure of myosin rod.
| Algorithms
|
||||||||
|---|---|---|---|---|---|---|---|---|
| Paircoil | Coils
|
|||||||
| MTK
|
MTIDK
|
|||||||
| Heptad position | Mutation | 14 | 21 | 28 | 14 | 21 | 28 | |
| d | A901G | − | +/− | − | − | +/− | − | − |
| L1793P | + | + | − | − | + | +/− | − | |
|
| ||||||||
| e | A1379T | − | − | +/− | − | − | +/− | − |
|
| ||||||||
| f | R1500P | + | + | + | + | + | + | − |
| R1500W | − | + | + | + | − | − | − | |
| R1845W | − | + | + | + | + | + | − | |
|
| ||||||||
| g | A1663P | + | + | + | + | + | + | +/− |
| R1712W | − | + | + | + | + | + | − | |
|
| ||||||||
| a | L1706P | + | + | + | + | + | + | + |
| K1354D | − | +/− | +/− | +/− | +/− | +/− | +/− | |
|
| ||||||||
| b | A1834M | − | − | +/− | +/− | − | +/− | +/− |
|
| ||||||||
| c | D1096Y | − | + | + | − | + | + | − |
| R1420W | − | + | + | + | − | − | − | |