Figure 4.

Identification of critical region of hBest1 required for Cav1.3 inhibition. A, Averaged Ba²⁺ current densities recorded during 50 ms test pulse at −10 mV for α₁1.3/β_1b/α₂δ either alone or with hBest1 or six hBest1 C-terminal fragments. The percentage of inhibition compared with control (α₁1.3/β_1b/α₂δ alone) is also shown. B, Averaged Ba²⁺ current densities recorded during 50 ms test pulse at −10 mV for α₁1.3/β_1b/α₂δ either alone or with hBest1 and six hBest1 N-terminal fragments. The right panels show the design for hBest1 fragments. Each averaged value represents the mean of 10–15 cells. *p < 0.05 for each group compared with Ca_V1.3 alone by two-way ANOVA.