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Transfection with plasmids driving expression of either GRIP1 or GRIP2 restores cerebellar LTD in GRIP1/2 double knock-out Purkinje cells. A, LTD induction by glutamate/depolarization pairing. Calibration: 50 pA, 1 s. GRIP1/2 DKO, GRIP1 plasmid, n = 7; GRIP1/2 DKO, GRIP2 plasmid, n = 8; GRIP1/2 DKO, GRIP1 PDZ5 mutant plasmid, n = 8. B, Chemical LTD of mEPSCs. n = 12 cells/group.
