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. Author manuscript; available in PMC: 2009 Aug 1.

Published in final edited form as: Biochim Biophys Acta. 2008 Mar 29;1783(8):1517–1528. doi: 10.1016/j.bbamcr.2008.03.011

Fig. 6 — A. HASMCs were treated with TNFα and either HATI (20 μM) or TSA (500ng/ml). Anti-NonO antibody was used in the ChIP assay. NonO-bound DNA was amplified in PCR using primers covering the region of −60 to +104bp in the P4Hα1 promoter. HATI treatment attenuated the TNF-induced NonO-P4Hα1 promoter interaction, whereas TSA treatment had no effect on the interaction. However, TSA treatment of control cells did slightly increase the interaction when compared with baseline level. B. and C. Expression of P4Hα1 in HASMCs treated with TNFα (100ng/ml) or JNK1 (100 μl) with or without HATI (20 μM) or TSA (500 ng/ml). Both HATI and TSA reversed the suppressive effects on P4Hα1 expression by the TNFα-JNK1 activation, although TSA-induced restoration was more complete than that induced by HATI. House-keeping control β-actin was regarded as 1, i.e. 100% expression.

Fig. 6 — A. HASMCs were treated with TNFα and either HATI (20 μM) or TSA (500ng/ml). Anti-NonO antibody was used in the ChIP assay. NonO-bound DNA was amplified in PCR using primers covering the region of −60 to +104bp in the P4Hα1 promoter. HATI treatment attenuated the TNF-induced NonO-P4Hα1 promoter interaction, whereas TSA treatment had no effect on the interaction. However, TSA treatment of control cells did slightly increase the interaction when compared with baseline level. B. and C. Expression of P4Hα1 in HASMCs treated with TNFα (100ng/ml) or JNK1 (100 μl) with or without HATI (20 μM) or TSA (500 ng/ml). Both HATI and TSA reversed the suppressive effects on P4Hα1 expression by the TNFα-JNK1 activation, although TSA-induced restoration was more complete than that induced by HATI. House-keeping control β-actin was regarded as 1, i.e. 100% expression.