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. Author manuscript; available in PMC: 2009 Nov 1.

Published in final edited form as: Hepatology. 2008 Nov;48(5):1558–1569. doi: 10.1002/hep.22499

Fig.4 — (A) The HEK 293 cells were transfected with wt GFP-Bsep, G238V and D482G. Twenty-four hours after transfection, the cells were incubated at 37°C for 24 hrs (control), 30°C for 24 hrs or treated with 10% glycerol for 24 hrs. GFP-Bsep was visualized by fluorescence microscopy. All the images were taken with the same microscopic setting. Bar=10 μm. (B) The lysates of the HEK 293 cells under these conditions were immunoblotted for GFP-Bsep and β-actin. ECL was used to visualize the protein of interest.

Fig.4 — (A) The HEK 293 cells were transfected with wt GFP-Bsep, G238V and D482G. Twenty-four hours after transfection, the cells were incubated at 37°C for 24 hrs (control), 30°C for 24 hrs or treated with 10% glycerol for 24 hrs. GFP-Bsep was visualized by fluorescence microscopy. All the images were taken with the same microscopic setting. Bar=10 μm. (B) The lysates of the HEK 293 cells under these conditions were immunoblotted for GFP-Bsep and β-actin. ECL was used to visualize the protein of interest.