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. Author manuscript; available in PMC: 2009 Aug 8.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jun 2;372(4):697–702. doi: 10.1016/j.bbrc.2008.05.132

Fig. 4. Biochemical analyses of MoPrP(23-231)-CFP and MoPrP(23-231)(Q167)- CFP.

Fig. 4

A.) Sarkosyl solubility assay. Soluble and pellet fractions of protein extracts containing different sarkosyl concentrations were prepared and analyzed as described in the materials and methods. A monoclonal PrP antibody (3F4) was used for immunoblot analysis. B.) Proteinase K digestion was performed as described in the Materials and Methods. A monoclonal GFP antibody was used for the immunoblot analysis. PrP-CFP: MoPrP(23-231)-CFP expressor. C.) Semi-denaturing agarose gel electrophoresis (SDAGE) assay. wt: MoPrP(23-231)-CFP expressor; Q167R: MoPrP(23-231)(Q167R)-CFP expressor; wt/Q167R: co-expressors of MoPrP(23-231)-CFP and MoPrP(Q167R)(23-231)-YFP.