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. Author manuscript; available in PMC: 2009 Jan 15.
Published in final edited form as: J Cell Sci. 2008 Jun 24;121(Pt 14):2360–2371. doi: 10.1242/jcs.014977

Figure 10. Dominant negative (DN) Eps15 does not inhibit fibronectin endocytosis.

Figure 10

DN Eps15 (A, C, E, G, I, K) or control Eps15 (B, D, F, H, J, L) were transiently expressed in FN-null MFs as described in Methods. (A-D) Transfected cells were seeded onto pre-assembled matrices and incubated for 24 h. Endocytosed fibronectin is shown in (A, B). (E-H) Short-term pulse chase experiments were performed with transfected cells. (E, F) show endocytosed fibronectin following 2 hr of chase. (I-L) The effect of DN and control Eps15 on transferrin receptor endocytosis is shown. Transfected cells were detected by GFP expression (C, D, G, H, K, L) and manually outlined. 15-20 cells were analyzed for each condition; representative images are shown. Scale bar, 10μm.