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. Author manuscript; available in PMC: 2009 Jan 15.
Published in final edited form as: J Cell Sci. 2008 Jun 24;121(Pt 14):2360–2371. doi: 10.1242/jcs.014977

Figure 6. Blocking of β1 integrins inhibits endocytosis of fibronectin from pre-assembled matrix.

Figure 6

FN-null MFs were incubated with either 30 μg/mL β1 inhibitory antibody (Ha2/5) or isotype control in suspension at room temperature for 30 minutes prior to seeding on pre-assembled TR-(A, B) or AF488 (C, D)-fibronectin matrix. Cells were cultured for 24 hours at 37°C, and were then either fixed for imaging assay (A, β1 inhibition; B, isotype control) or processed for flow cytometry to quantitate internalized fibronectin (C, D). The numbers over the peaks in C are the MFI of internalized AF 488-fibronectin. Graph in D shows fold change relative to the MFI of endocytosed AF488-fibronectin in cells treated with isotype control IgM, which was set equal to 1 (n=4, mean ± s.d.). Scale bar, 10μm.