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. Author manuscript; available in PMC: 2009 Jan 15.
Published in final edited form as: J Cell Sci. 2008 Jun 24;121(Pt 14):2360–2371. doi: 10.1242/jcs.014977

Figure 8. Blocking of α5 integrin partially inhibits fibronectin endocytosis.

Figure 8

FN-null MFs were incubated with 50 μg/mL α5 inhibitory antibodies (5H10-27), or 100 μg/mL αv inhibitory antibodies (H9.2B8), or isotype control IgG at 4°C for 30 minutes. 10 μg/mL TR-fibronectin (A-D) or AF488-fibronectin (E) was added to the media. After 2 hours chase, cells were either fixed for imaging analysis (A, α5 inhibition; B, isotype control of α5; C, αv inhibition; D, isotype control for αv), or processed for flow cytometry (E) to quantitate internalized fibronectin. The numbers over the peaks in E are the MFI of internalized AF 488-fibronectin. Scale bar, 10μm.