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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Protein Expr Purif. 2008 Aug 7;62(2):216–222. doi: 10.1016/j.pep.2008.07.014

Figure 6.

Figure 6

Kinetics of GPx4-catalyzed 7α-OOH reduction. Each 0.5 mL reaction mixture contained 3 mM GSH, 1 mM EDTA, 0.1 mM DFO, 0.1% Triton X-100, 25 μM 7α-OOH, and GPx4 in PBS at 37 °C. GPx4 concentration was either 0.9 μg/mL (△) or 1.8 μg/mL (▲). First-order plots of 7α-OOH decay as a function of incubation time are shown. Data from a control lacking GPx4 are plotted alongside (○). Values are means from duplicate experiments.