Figure 3.

Correction in Hz to be applied to the (D + J) value as measured from the E.COSY spectra, as a function of (D + J) and R₁^Hα. Opposite sign corrections of the same magnitude are required for interactions where (D + J) < 0. Corrections were derived using peak picking on multiplets simulated using eq 9, assuming H^N Lorentzian (left) or Gaussian (right) line shapes with line width at half height of 35 Hz, and H^α spin flips occurring between the end of the C^α evolution period and the start of direct detection (28.8 ms).