Figure 3.
Effects of RBP2 on human RalR1. (A) Microsomes (108 μg protein) prepared from RalR1/CHO cells were incubated at 37 °C for 1 h with various concentrations of either free- or RBP2 bound-retinal (holo-RBP2) as indicated. Production of retinol was measured by HPLC. (B) In the above assay conditions, increasing concentrations of apo-RBP2 was added to a constant level of free retinal (2 μM), and the production of retinol was measured by HPLC.