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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: J Mol Biol. 2008 Jan 11;377(3):774–789. doi: 10.1016/j.jmb.2007.12.077

Figure 4. Polypeptide composition of RNAP affinity purified from phiEco32-infected cells.

Figure 4

A. The β′-4PrA fusion protein, and co-isolating proteins, was affinity purified from E. coli 55 infected with phiEco32 (lane 1; control), E. coli 55rpoC::4PrA (lane 2), and E. coli 55rpoC::4PrA + phiEco32 (lane 3). The samples were analyzed by SDS-PAGE and bands due to proteins present in lane 3, but absent from lanes 1 and 2, were identified by MALDI MS.

B. NSAF values for proteins identified by MudPIT in RNAP samples affinity purified from both phiEco32-infected and uninfected cells are shown.