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. Author manuscript; available in PMC: 2009 May 6.
Published in final edited form as: Curr Biol. 2008 May 6;18(9):641–649. doi: 10.1016/j.cub.2008.04.017

Fig. 6. CARP-2 interacts with RIP kinase domain.

Fig. 6

(A). CARP-2 binds RIP kinase domain. Extracts from 293T cells expressing FLAG tagged RIP kinase domain (KD) or without kinase domain (ΔKD) were incubated with extracts from cells expressing vector or wild type V5-CARP-2. RIP was immuno-precipitated and probed for CARP-2.

(B). Kinase domain is essential for CARP-2 mediated RIP loss. Extracts from HeLa cells transfected with pcDNA3-nV5-RIP Full length (FL) or ΔKD together with CARP-2 were probed for indicated proteins.

(C). Kinase domain is essential for CARP-2 mediated RIP degradation. 293T cells transfected with pcDNA3-nV5 -RIPΔKD and pFLAG-CMV-CARP2 variants were analyzed by procedure described in figure 5C.

(D). RIP kinase domain negatively regulates TNF-induced NF-κB activation. Extracts of medium (Med) or TNF treated RIP−/− MEFs transfected with indicated constructs in pMSCV vector along with reporter plasmids were assayed for the reporter activities.