Table 3.
Temperature Dependence of the ITC-Derived Parameters for the Binding of HXDV and HXLV-AC to hTel24-NMR in the presence of 50 mM K+a
| Compound | Temperature (°C) | K (M−1) | ΔH (kcal/mol) | N |
|---|---|---|---|---|
| HXDV | 25 | (3.0 ± 0.4) × 105 | −1.7 ± 0.1 | 2.2 ± 0.1 |
| HXDV | 30 | (2.8 ± 0.4) × 105 | −2.4 ± 0.1 | 2.0 ± 0.1 |
| HXDV | 35 | (3.5 ± 0.3) × 105 | −3.1 ± 0.1 | 1.9 ± 0.1 |
| HXDV | 40 | (6.9 ± 0.5) × 105 | −3.8 ± 0.1 | 1.8 ± 0.1 |
| HXLV-AC | 25 | (5.5 ± 0.6) × 105 | −2.1 ± 0.1 | 1.9 ± 0.1 |
| HXLV-AC | 30 | (4.2 ± 0.5) × 105 | −2.8 ± 0.1 | 1.9 ± 0.1 |
| HXLV-AC | 35 | (5.4 ± 0.5) × 105 | −3.4 ± 0.1 | 1.8 ± 0.1 |
| HXLV-AC | 40 | (1.1 ± 0.1) × 106 | −4.0 ± 0.1 | 1.7 ± 0.1 |
a
Buffer conditions were as described in the footnote to Table 2. The listed values of K, ΔH, and N were derived from fits of ITC profiles (as shown in Fig. 7 for HXDV) with a model one set of binding sites. The indicated uncertainties reflect the standard deviations of the experimental data points from the fitted curves.