Skip to main content
. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Immunity. 2007 Dec 27;28(1):29–39. doi: 10.1016/j.immuni.2007.11.016

Figure 5. RORα and RORγt synergizes in promoting TH17 differentiation.

Figure 5

Naïve OT-II CD4+ T cells were activated with OVA peptide-pulsed splenic APCs under the Neutral (anti-IL-4 and anti-IFN-γ); iTreg (inducible regulatory T cells, TGF-β, anti-IL-4 and anti-IFN-γ), TH1 or TH2 conditions and co-infected with two bicistronic retroviruses expressing RORα-GFP or GFP vector and RORγt-hCD2 or hCD2 vector. Cytokine- or Foxp3-expressing cells were assessed by intracellular staining. Data shown are gated on GFP+hCD2+ cells. The experiments were repeated at least twice with consistent results.