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Image Processing Algorithm. Outline and background colors of components correspond to the bitplane colors as described in the text. For a detailed description, please refer to the following subsections in the semiautomated histomorphometry section of Materials & Methods: A, Thresholding and manual fiber debris elimination; B, Axon definition and manual feature elimination; C and D, Fiber separation and further delineation of nonmyelinated profiles; E, Mathematical morphometry.
