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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: J Neurosci Methods. 2007 Jun 30;166(1):116–124. doi: 10.1016/j.jneumeth.2007.06.018

Figure 2.

Figure 2

A. Original gray scale image. B. Red myelin bitplane. During the initial manual myelin thresholding step, all gray level profiles are colored red. C. Axon bitplane. All features with myelin thresholded are colored green. D. Manual elimination of non-axon features initially colored green by the program. Black arrows demonstrate areas where incorrect axon identification is manually eliminated.