Fig. 8.
Aβ42 specific antibody from a vaccinated monkey. The monkey was vaccinated with the gene-gun delivered Aβ42 gene vaccine in 4 monthly intervals with 8 µg DNA and the serum obtained 2 months after the last immunization. A. ELISA assay shows anti-Aβ42 titer reached 1:20,000. B. Western blot shows Aβ42 gene vaccination in monkey elicits a specific antibody response against Aβ42 and also against Aβ1–16, Aβ17–28, and Aβ29–42. Lanes 1, 2 and 3 were loaded with GST fused to Aβ1–16, Aβ17–28 or Aβ29–42, respectively. Preimmune or immune serum was then added.