Fig. 4.

Clone 4 CD8⁺ T cell-mediated autoimmunity in lymphopenic InsHA mice depends on antigen-specific CD4 help. Irradiated InsHA mice were injected with 3.5 × 10⁶ Thy1.1⁺ Clone 4 CD8⁺ T cells together with either 3.5 × 10⁶ InsHA Thy1.1⁺ CD4⁺ T cells or 3.5 × 10⁶ HNT Thy1.1⁺ CD4⁺ T cells. Mice were killed on day 20 after transfer and proliferation of gated CD4⁺ Thy1.1⁺ and CD8⁺ Thy1.1⁺ lymphocytes cells in pLN, and spleen was assessed as a function of CFSE fluorescence (Top). Expression of CD62L and CD25 on gated CD8⁺ Thy1.1⁺ lymphocytes was assessed and the percentages of highly proliferating donor CD62L^lo cells and CD25⁺ cells are indicated in each condition. Data from 1 representative experiment of 4 are presented.