Table 1.
CD8+ and CD4+ T cells cooperate to induce autoimmunity under lymphopenic conditions
| Cell transfer* | Number of cells | Diabetes incidence† | Diabetes onset‡ |
|---|---|---|---|
| Recipient, irradiated InsHA | |||
| Clone 4 CD8+ | 2 × 106 | 0/11 | — |
| 3.5 × 106 | 0/6 | — | |
| 5 × 106 | 1/21 | d 12 | |
| 7 × 106 | 0/5 | — | |
| 10 × 106 | 0/6 | — | |
| HNT CD4+ | 2 × 106 | 0/10 | — |
| 3.5 × 106 | 1/8 | d 12 | |
| 5 × 106 | 0/18 | — | |
| 7 × 106 | 0/5 | — | |
| 10 × 106 | 2/10 | d 21 | |
| Clone 4 CD8+ + HNT CD4+ | 2 × 106 + 2 × 106 | 7/7 | d 14 ± 4 |
| 3.5 × 106 + 3.5 × 106 | 13/15 | d 14 ± 3 | |
| 5 × 106 + 5 × 106 | 14/14 | d 11 ± 1 | |
| Clone 4 CD8+ + DO11.10 CD4+ | 3.5 × 106 + 3.5 × 106 | 0/11 | — |
| Clone 4 CD8+ + InsHA CD4+ | 3.5 × 106 + 3.5 × 106 | 0/8 | — |
| Recipient, nonirradiated InsHA | |||
| Clone 4 CD8+ + HNT CD4+ | 5 × 106 + 5 × 106 | 0/8 | — |
*Purified transgenic CD8+ and/or CD4+ T cells, as indicated, were injected into either irradiated or nonirradiated InsHA mice.
†The onset of autoimmunity was evaluated by measuring blood glucose levels. Mice were followed over a 1 month period and were considered diabetic when levels were above 300 mg/dL in 2 consecutive measurements.
‡The day of disease onset (d) is indicated when applicable.